Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator

Data underlying the figures in the publication: Lopez-Morales, J. et al. “Titrating Avidity of Yeast-Displayed Proteins Using a Transcriptional Regulator” ACS Synth. Biol. 2023, 12, 419-31, https://doi.org/10.1021/acssynbio.2c00351 Table of Contents Fig1_subplots_EMpty pJL100 full atc range analysis.xlsx: Flow cytometry data of displaying fraction and median fluorescence for Fig. 1c and d. Fig2_subplots_20.08.05 tit disp GOx ABTS.xlsx: Flow cytometry data of displaying fraction and median fluorescence of Gox variants; GOX enzymatic activity assay data for Fig. 2 Fig3_subplots_ SDA data for sharing.xlsx and disks + sup data plot.pptx: Spinning disk assay data and micrographs for Fig. 3. Fig4_ subplots_20220421 YTD affibody aTc vs Gal titration curves.xlsx and 20220423 Gal good volume.xlsx: Flow cytometry data and PDL1 titration on yeast data for Fig. 4. Supplementary_Gal comparison_ Gal time point-2.xlsx: Flow cytometry data for comparing the YTD system to the standard yeast surface display for sup. Fig. S1. Supplementary_Fig S3_Titrated Affibody Kd analysis.xlsx and Simulations.pzf: ICE tables and simulation spreadsheet for sup. Fig. S3.