Brain microvessels ontogeny-ERI-INSERM28

Enriched forebrain microvessels from P5, P10 and adult mice. Transcript comparisons identified 5873 genes with FC>2 between ages and 93 pathways. The observations were confronted to proteome study using NanoLS-MS/MS and deposited on proteomeXchange platform under # PXD001718. The 2 techniques converge towards extracellular matrix, cell adhesion and junction pathways, indicating early changes in development. The difference are examined as putative structural basis of very preterm intracerbral hemorrhage, 3 age comparisons of 5 days, 10 days and adult brain microvessels, 2 replicates, Agilent Whole Mouse Genome Oligo 4x44K Microarray本数据集包含取自出生后第5天（P5）、第10天（P10）及成年小鼠的富集前脑微血管。通过转录组比较分析，共鉴定出5873个不同年龄间倍数变化（FC, Fold Change）大于2的基因，以及93条相关通路。将上述观测结果与基于纳米液相色谱-串联质谱（NanoLC-MS/MS）的蛋白质组学研究进行比对验证，并将相关数据上传至ProteomeXchange平台（ProteomeXchange），登录编号为PXD001718。两种技术的分析结果均聚焦于细胞外基质、细胞黏附及细胞连接通路，提示小鼠脑微血管发育过程中的早期分子变化。本研究将上述表达差异作为极早产脑内出血（intracerebral hemorrhage）的潜在结构基础开展分析；实验设置3个年龄组别（出生后5天、10天及成年小鼠脑微血管），包含2次生物学重复，采用安捷伦（Agilent）全小鼠基因组寡核苷酸4x44K微阵列（Microarray）完成检测。