An ATAC-seq atlas of chromatin accessibility in mouse tissues

Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) is a fundamental epigenomics approach and has been widely used in profiling the chromatin accessibility dynamics in mammalian species, such as human and mouse. However, a comprehensive reference ATAC-seq dataset source of adult mouse tissues is still lacking, hampering the discovery of tissue-specific accessible elements. Here, we report on a mouse ATAC-seq atlas by producing a total of 66 ATAC-seq profiles from 20 primary tissues of both male and female mice. The ATAC-seq read enrichment, fragment size distribution and reproducibility between replicates revealed high quality of the full datasets. We finally identified a sum of 296,574 accessible elements. We further identified key transcription factors specific to distinct tissues and found that the enrichment of each transcription factor can well reflect the developmental distance across tissues. In summary, our study provided an important resource on mouse epigenome and will be of high-value in various studies such as development, cell reprogramming and genetic diseases.