File S1 - miR-106a-5p Inhibits the Proliferation and Migration of Astrocytoma Cells and Promotes Apoptosis by Targeting FASTK

Supporting information file including Figures S1–S3 and Table S1. Figure S1. Relative expression of miR-106b-5p after miR-106a-5p transfection. U251 cells were seeded into 6-well plates and transfected the following day using Lipofectamine 2000. For each well, 100 pmol of pre-ncRNA, pre-miR-106a-5p, anti-ncRNA or anti-miR-106a-5p was transfected. The intercellular levels of miR-106b-5p were evaluated by qRT-PCR at 24 h after transfection. For comparison, the expression levels of miR-106b-5p in pre-ncRNA- or anti-ncRNA-transfected cells were arbitrarily set at 1. The results are presented as the mean ± SD of three independent experiments. Figure S2. Evaluation of the absolute expression level of miR-106a-5p in astrocytoma cells. Either 10−4, 10−3, 10−2, 10−1, 100, 101, or 102 fmol of single strand miR-106a-5p synthesized by TaKaRa (Dalian, China) were assessed by qRT-PCR assay. The resulting Ct values were plotted versus the log10 of the amount of input miR-106a-5p. Then the absolute amount of miR-106a-5p in astrocytoma cells was calculated by referring to the standard curve. Figure S3. The role of miR-106a-5p and FASTK in cell apoptosis in U87 cells. U87 cells were transfected with equal concentrations of pre-ncRNA, pre-miR-106a-5p, si-NC and si-FASTK. The experiment was repeated three times, and representative data are shown. Table S1. Summary of the demographic and clinical features of the 84 astrocytoma samples and the 20 NAT samples (DOC)