Proteomic Analysis of Human Fetal Atria and Ventricle

In this study we carried out a mass
spectrometry-based proteome
analysis of human fetal atria and ventricles. Heart protein lysates
were analyzed on the Q-Exactive mass spectrometer in biological triplicates.
Protein identification using MaxQuant yielded a total of 2754 atrial
protein groups (91%) and 2825 ventricular protein groups (83%) in
at least 2 of the 3 runs with ≥2 unique peptides. Statistical
analyses using fold-enrichment (>2) and p-values
(≤0.05) selected chamber-enriched atrial (134) and ventricular
(81) protein groups. Several previously characterized cardiac chamber-enriched
proteins were identified in this study including atrial isoform of
myosin light chain 2 (MYL7), atrial natriuretic peptide (NPPA), connexin
40 (GJA5), and peptidylglycine alpha-amidating monooxygenase (PAM)
for atria, and ventricular isoforms of myosin light chains (MYL2 and
MYL3), myosin heavy chain 7 (MYH7), and connexin 43 (GJA1) for ventricle.
Our data was compared to in-house generated and publicly available
human microarrays, several human cardiac proteomes, and phenotype
ontology databases.