Murine Bone Marrow Erythroid Cells Have Two Branches of Differentiation Defined by the Presence of CD45 and a Different Immune Transcriptome Than Fetal Liver Erythroid Cells

Ter-119 is a better murine erythroid selection marker than CD71 for bulk immunomics, murine bone marrow and fetal liver erythroid cells have different antimicrobial immune transcriptome signatures, murine bone marrow erythropoiesis is comprised of two branches In this study, we decided to benchmark erythroid cells’ selection and enrichment markers – CD71 and Ter-119 via bulk immune transcriptomics by Nanostring and perform bulk immune transcriptome profiling of Ter-119+ erythroid cells from the bone marrow and the fetal liver by Nanostring. We profiled total RNA from either CD71+ erythroid cells (CECs) or Ter-119+ erythroid cells from the bone marrow and Ter-119+ erythroid cells from the fetal liver on NanoString Sprint Platform using Mouse Immunology V1 panel. We performed mRNA profiling for 561 target genes, 14 housekeeping genes, 8 negative and 6 positive spike-in controls