Evaluation of the effects of a maternal high-fat diet on myelination impairment in adolescent rat offspring using immunofluorescence staining to visualize specific markers of oligodendrocyte lineage cells (CC-1, NG2, Olig2)

Evaluation of the effects of a maternal high-fat diet (HFD) on myelination impairment in adolescent rat offspring (male and female, 28 PND) using immunofluorescence staining to visualize specific markers of oligodendrocyte lineage cells (CC-1, NG2, Olig2) compared to offspring, whose mother's were fed a standard diet (SD). The following structures were visualized prelimbic and infralimbic cortex (PREL), cingulate cortex (CIN), and motor cortex (MOT) according to Rat Brain Atlas.NG2- magenta channel (Alexa Fluor 647)CC-1- cyan channel (Alexa Fluor 488)Olig2- yellow channel (Alexa Fluor 568)Equipment: a scanning confocal microscope (Leica SP8 WLL) Magnification: 20× Objective: HC PL APO 20x NA=0.75 CS2Software: FIJI open-source software or LASX (.lif) File type: .lif, .tiff