Lymphocyte Subsets Reference Ranges in Omanis

The purpose of our study is to identify the reference ranges for healthy Omani donors for T-cell, B-cell and NK-cell lymphocyte subsets with a comprehensive and informative panel of MoAbs with the CD45/SSC log gating strategy, in both males and females in a broad age spectrum. Internal quality control procedures were used to assess instrument parameters and assure accurate results. Daily calibration of the flow cytometer was performed using Flow-check and Flow-set fluorospheres (Beckman Coulter) for optical laser alignment and optimal hydrodynamic focussing settings respectively. An external quality assurance procedure was also implemented through participation in a performance-monitoring network operated by The Royal College of Pathologists of Australasia Quality Assurance (RCPA) programme.