Uterine-specific SIRT1 deficiency confers premature uterine aging and impairs invasion and spacing of blastocyst and stromal cell decidualization in mice.

We demonstrate that Sirtuin 1 (SIRT1) is an indispensable age-related regulator of invasion and spacing of blastocysts, as well as decidualization of stromal cells. Here, we report the sequencing of the uterine transcriptome of control and conditional knock-out of the Sirtuin 1 (SIRT1) at day 3.5 of murine pregnancy. Overall design: Mice with uterine-specific ablation of Sirt1 (Sirt1d/d) and littermate controls (Sirt1f/f) were mated to male mice of proven fertility. Upon the presence of the post-coital plug (Gestational day 0.5, GD0.5), mice were sacrificed 3 days later (GD3.5) and uterine horns (embryo were flushed out) were harvested and frozen. RNA was isolated from the uterus. Six biological samples per genotype were sequenced for differential gene expression analysis.