Perinatal exposure to lead and DEHP change DNA methylation profile in mouse liver and blood

Purpose: Then goal of this study id to investigate the effects of perinatal lead (32 ppm in maternal drinking water) and DEHP (25 mg per kg of chow) exposure on liver and blood DNA methylation in both PND21 and 5-month male and female mice. Methods: Enhanced reduced-representation bisulfite sequencing (ERRBS) was used to assess DNA methylation in PND21 and 5-month old DEHP-exposed, lead-exposed and control mice. Sex-specific differential methylaton analysis by DEHP and lead exposure was conducted using MethylSig R package. Results: We observed hundreds to thousands of stably modified, sex-specific differentially methylated regions in blood and liver of DEHP-exposed and lead-exposed animals at both PND21 and 5 months. A total of 151 DNA methylation profiles of PND21 and 5-month mouse blood and liver with DEHP exposure, lead exposure or no exposure, measured by Enhanced Reduced Representation Bisulfite Sequencing (ERRBS)