Exome sequencing of donor hematopoietic cells from Sipa1+/+ and Sipa1-/- recipient bone marrow and spleen

The exome sequencing was performed on the donor cells (CD45.1+) from bone marrow (BM) and spleen (SPL) of either lethally irradiated (experiment 1: HBM-WT and HBM-KO) or sublethally irradiated (experiment 2: IBM-WT, IBM-KO and ISPL-WT, ISPL-KO) Sipa1+/+ and Sipa1-/- recipient mice (CD45.2+) 7 or 9 months after transplantation. The donor CD45.1+ cells were sorted by FACS from spleen and BM of same recipients.Genomic DNA was extracted from the sorted cells and processed using SureSelect XT2 Target Enrichment System for Illumina Multiplexed Sequencing Protocol (Agilent Technologies). The libraries were sequenced on a NextSeq 500/550 using High output kit v2 (150 cycles) (Illumina).