CIeaD: A Complementary CID and EAD Mass Spectral Library for Phytochemicals

Plant metabolomics
faces major challenges in metabolite identification
due to the structural diversity of plant metabolites and limited coverage
in existing spectral libraries. To address this, we developed CIeaD
(Collision-Induced and Electron-Activated Dissociation), an open-access
plant metabolite spectral library containing complementary CID and
EAD spectra. The library includes curated high-resolution spectra
for 2,305 phytochemicals across major metabolite classes, acquired
in both positive and negative modes with a dual fragmentation mechanism
to capture a wide range of diagnostic ions. CIeaD library is provided
in multiple formats and can be accessed at https://www.moleculardetective.org/Links.html.