Single cell RNA sequencing profile of 60 day old human iPSC-derived midbrain organoids from healthy individual, Parkinson's disease patient (Miro1 p.R272Q mutation) and its isogenic control

Use of patient specific models, such as midbrain organoids, have shown to be valuable tools to models Parkinson's disease as well as to identify novel pathological cellular and molecular mechanisms. In here, we used single cell RNA sequencing analysis of 60 day old midbrain organoids obtained from a Parkinson's disease patient carrying the p.R272Q Miro1 mutation, its isogenic control and a healthy control to explore the effect of RhoT1 gene in different cellular populations present within the midbrain organoids (e.g. dopaminergic neurons, astrocytes) and the subsequent effect on the PD pathology. Overall design: Midbrain organoids generated from iPSC obtained from a Parkinson's disease patient carrying the Miro1 p.R272Q mutation, its respective isogenic control and from a healthy age and sex-matched donor were used. The midbrain organoids were culture for 60 days and then, dissociated into single cells and used for scRNA-seq analysis.