METHOD FOR IMPROVING EXPRESSION LEVEL OF RECOMBINANT PROTEIN IN ENDOSPERM BIOREACTOR
收藏国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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资源简介：
Provided is a method for improving the expression level of a recombinant protein in an endosperm bioreactor. By using storage glutelin mutant (LGC-1) hybridization or gene editing (TALEN technology and CripsrCas9) technology， the method carries out the targeted knockout of a gene of an endogenous storage protein， reduces the content of the endogenous storage protein， relieves the endoplasmic reticulum stress， and improves the transport efficiency of the recombinant protein in endosperm cells， thereby improving the expression of the recombinant protein in endosperm cells and achieving the increased yield of rice lines with the recombinant protein and a significant improvement in the expression level of an exogenous recombinant protein under the Oryz HiEXP technological platform.
提供机构：
国家林业和草原科学数据中心
创建时间：
2023-02-12
