Device comparison.

A comparison of selected commercial and academic devices that have demonstrated single-cell time-lapse cultures for budding yeast. 1 Numbers reported refer to the original, cited papers and represent reported data, not theoretical maximum numbers. Some devices, such as the Cellasic, have many citations in which larger numbers of cells may have been recorded and for longer periods. The number of cells refers to those cells originally loaded and excludes daughter cells born in the device except for the Microfluidic imaging matrix and Microchemostat arrays2, for which numbers of loaded cells were not reported. Note the trade off between the intervals between imaging frames and the number of cells recorded, and that the density of cells in view has not been reported for most devices. Note also that larger cell numbers can be imaged for shorter times in devices that suffer loss of mother cells over time, such as the ALCATRAS devices. We have assumed that all reported datasets come from a single experiment with a single device.