Data for heme fractionation of PPQ-treated and untreated parasites with variable plasmepsin copy numbers (Fig 3A–C).

Different heme species were extracted from parasites by subsequent cellular fractionation steps. Tightly synchronized KH001_053G10 and KH001_053G8 ring-stage parasites were exposed to various PPQ concentrations for 32 h. The amount of iron species (Fe) per sample was estimated based on the standard curve run for each biological replicate and the percentages for each species per sample were calculated. Shown is each value from three biological replicates run in quadruplicate with the average and SD of percentage of hemozoin Fe, hemoglobin, and free heme Fe. Statistical comparisons of the drug-treated lines to their untreated controls were performed using two-tailed unpaired Student’s t-tests *p p (XLSX)