ChIP-seq analysis for STAT3 targets downstream of LIF signaling in liver ischemia-reperfusion injury

LIF is a pleotropic cytokine and its role in liver ischemia-reperfusion injury (IRI) remains unexplored. Utilizing wildtype (WT) and myeloid cell-specific knockout (KO) mice, we demonstrate that the transcription of LIF is activated in myeloid cells and promotes LIFR-induced STAT3 activation in hepatocytes during liver IRI. To understand the downstream targets of STAT3, we performed ChIP-seq analysis. Overall design: WT and KO mice underwent 1 h ischemia/6 h reperfusion injury and liver samples were collected. The samples were then analyzed with STAT3 ChIP-seq.