RNA sequencing analysis in ESCC cells with Drp1 overexpression or TFAM deficiency

Mitochondrial homeostasis is important for cell metabolism, growth, proliferation, and immune responses. The critical regulator for mitochondrial homeostasis, Drp1 and TFAM are frequently abnormal expression in many cancers and is closely implicated in tumorigenesis. Here, we found that Drp1 high expression or TFAM low expression is correlated with poor overall survival of ESCC patients. However, the underling mechanism by Drp1 or TFAM influence tumor progression is largely unknown, especially in esophageal squamous cell carcinoma (ESCC). To investigate the underling mechanisms of Drp1 overexpression or TFAM deficiency-mediated ESCC progression, transcriptome profiling was performed by RNA sequencing analysis in ESCC cells with Drp1 overexpression or TFAM knockdown. Human ESCC cell lines KYSE-30 with Drp1 overexpression or TFAM deficiency were established by stable transfection with pcDNA3.1(+)-Drp1 or pSilencer3.1-H1 neo-shTFAM. Differential gene expression analysis after Drp1 overexpression or TFAM knockdown are performed by RNA sequencing analysis in ESCC cells when compared to control KYSE-30 cell lines.