Single-nucleus RNA sequencing of the prefrontal cortex from mice lacking glutaminase 1 in CamKIIa-positive neurons and control mice

Autism spectrum disorder (ASD) is a heterogeneous group of disorders with shared diagnostic phenotypes, such as reduced interest in social interaction and communication, and increased stereotyped or restricted interests and behaviors. Little is known about the involvement of alterations of glutaminase 1 (Gls1, an enzyme that catalyzes the hydrolysis of glutamine into glutamate) in the pathogenesis of ASD. To investigate the role of Gls1 in forebrain neurons , we generated conditional mouse mutants with loss of Gls1 in forebrain CamKIIa-Cre positive neurons by crossing Gls1flox/flox mice with CamKIIacre mice. Overall design: Conditional mouse mutants with loss of Gls1 in forebrain CamKIIa-Cre positive neurons were generated by crossing Gls1flox/flox mice with CamKIIacre mice. Mosaic homozygous Gls1 mutant (CamKIIaCre;Gls1flox/flox) mice were not viable. Only mosaic heterozygous Gls1 mice (CamKIIaCre;Gls1+/flox mice, referred to as Gls1_CamKIIaCre mice) and their littermate CamKIIaCre;Gls1+/+ mice (referred to as control mice) were used in this study. Cells from the prefrontal cortex of both groups were isolated for Single-Nucleus RNA sequencing. Three animals were included for each group.