RNA-seq gene expression profiling of livers from wild-type male and female mice, and from mice with a Double Knockout (DKO) of Ezh1 and Ezh2.

PolyA-selected RNA isolated from livers of adult male and female mice with a combined loss of EZH1 and EZH2 in hepatocytes was analyzed by RNA-seq. The DKO mouse model used in these analyses is a global deletion of Ezh1 and hepatocyte-specific deletion of Ezh2, and is described in Bae WK, Kang K, Yu JH, Yoo KH, Factor VM, Kaji K, Matter M, Thorgeirsson S, Hennighausen L. FASEB J. 2015 May;29(5):1653-62. doi: 10.1096/fj.14-261537. PMID: 25477280 Liver RNA, polyA-selected, was isolated from 7 week old male and female DKO mice (Ezh1-/-, Ezh2-f/f_Alb-Cre) and their floxed (f) controls. RNA samples from n=3 mouse livers were pooled to make each of 3 biological replicate libraries per sex and genotype. AC, Albumin-Cre本研究通过RNA测序（RNA-seq）技术，分析了肝细胞中EZH1与EZH2联合缺失的成年雌雄小鼠肝脏中提取的聚腺苷酸化筛选RNA（PolyA-selected RNA）。本研究使用的双敲除（DKO）小鼠模型为Ezh1全基因敲除联合肝细胞特异性Ezh2敲除，该模型的构建细节参见文献：Bae WK, Kang K, Yu JH, Yoo KH, Factor VM, Kaji K, Matter M, Thorgeirsson S, Hennighausen L. FASEB J. 2015 May;29(5):1653-62. doi: 10.1096/fj.14-261537. PMID: 25477280。实验所用的聚腺苷酸化筛选RNA提取自7周龄的雌雄双敲除小鼠（基因型为Ezh1-/-, Ezh2-f/f_Alb-Cre）及其条件性敲除对照（floxed，缩写为f）。每个性别与基因型组取3只小鼠的肝脏RNA样本混合，以构建对应组的3个生物学重复文库。注：AC即白蛋白-Cre（Albumin-Cre）