C1R cells expressing different MICA alleles were incubated with PBMCs from healthy donors for 4 hours. A range of doses of MICAB1-Fc silent or isotype control mAb was added to the cells. The variation
We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X