Transcriptome profiling of Bma1 knock out mouse by RNASeq

BMAL1 directs the circadian expression of many genes implicated in cartilage matrix homeostasis including those involved in catabolic/anabolic and apoptosis pathways. We maintained mice in 12:12 light and dark cycle and sequenced mRNA from both wild type and BMAL1 knockout mice from time point at 5am, 9am, 5pm and 9pm.BMAL1可介导诸多参与软骨基质稳态的基因的节律性表达，其中涵盖了与分解代谢、合成代谢及细胞凋亡通路相关的基因。本研究将小鼠饲养于12小时光照/12小时黑暗的光周期环境中，并分别于凌晨5点、上午9点、下午5点及晚上9点四个时间点，提取野生型与BMAL1基因敲除小鼠的信使RNA（mRNA）进行测序。