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Data from: Sex-specific dispersal patterns of the threatened Northern River Shark

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Research Data Australia2025-12-20 收录
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https://researchdata.edu.au/data-from-sex-river-shark/3653125
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Full mitochondrial genomes of 384 Northern River Sharks, Glyphis garricki, and their associated metadata are stored here. The genetic data was produced to investigate the sex-specific dispersal behaviour of the species in Northern Australia and Papua New Guinea.\nLineage: The full mitochondrial genomes were amplified with two primer pairs. The amplicons were fragmented and barcoded with the Nextera XT DNA Sample Preparation kits and 96 sample Nextera Index kits. Libraries were then pooled and sequenced on a Miseq desktop sequencer using the 2x250 bp paired-end reads MiSeq reagent kit v2.\n\nThe SNP data were created by Feutry et al. (2020), following the DArTseq protocol (Diversity Arrays Technologies, Canberra).

本数据集收录了384尾北河鲨(Glyphis garricki,Northern River Shark)的完整线粒体基因组(mitochondrial genomes)及其相关元数据。本套遗传数据的生成旨在探究该物种在澳大利亚北部与巴布亚新几内亚境内的性别特异性扩散行为。 实验流程:研究团队采用两对引物对扩增获取完整线粒体基因组。扩增产物经Nextera XT DNA样本制备试剂盒与96样本Nextera索引试剂盒进行片段化与条形码标记。随后将文库混合,使用MiSeq桌面测序仪搭配2×250 bp双端读取MiSeq试剂试剂盒v2完成测序。 单核苷酸多态性(SNP)数据由Feutry等(2020)依据DArTseq流程(澳大利亚堪培拉多样性阵列技术公司,Diversity Arrays Technologies)生成完成。
提供机构:
Commonwealth Scientific and Industrial Research Organisation
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