Curcumin improves glycolipid metabolism through regulating peroxisome proliferator activated receptor γ signalling pathway in high-fat diet-induced obese mice and 3T3-L1 adipocytes

Curcumin, an active component derived from the Curcuma longa L. which is a traditional Chinese medicine that is widely used for treating metabolic diseases through regulating different molecular pathway. Here, in this study, we aimed to comprehensively investigate the effects of curcumin on glycolipid metabolism in vivo and in vitro and then determine the underlying mechanism. Male C57BL/6J obese mice and 3T3-L1 adipocytes were used in vivo and in vitro study, respectively. Our results demonstrated that treatment with curcumin for 8 weeks decreased body weight, fat mass and serum lipid profiles. Meanwhile, it lowered fasting blood glucose and increased the insulin sensitivity in high-fat diet induced obese mice. In addition, curcumin stimulated lipolysis and improve glycolipid through upregulating the expressions of ATGL, HSL, PPARγ, C/EBPα and PPARα in adipose tissue of the mice. In differentiated 3T3-L1 cells, curcumin reduced glycerol release and increased glucose uptake via upregula...姜黄素（Curcumin）是姜黄（Curcuma longa L.）的活性成分，而姜黄是一味传统中药，其通过调控多条分子通路广泛用于代谢性疾病的治疗。本研究旨在全面探究姜黄素在体内外对糖脂代谢的影响，并阐明其潜在作用机制。本研究分别选用雄性C57BL/6J肥胖小鼠与3T3-L1脂肪细胞作为体内、体外实验的研究模型。实验结果表明，连续8周给予姜黄素治疗可降低高脂饮食诱导肥胖小鼠的体重、脂肪量及血清脂质水平；同时可降低空腹血糖，提升胰岛素敏感性。此外，姜黄素可通过上调小鼠脂肪组织中脂肪甘油三酯脂肪酶（ATGL）、激素敏感性脂肪酶（HSL）、过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT增强子结合蛋白α（C/EBPα）及过氧化物酶体增殖物激活受体α（PPARα）的表达，促进脂肪分解并改善糖脂代谢。在分化成熟的3T3-L1细胞中，姜黄素可通过上调……