Effects of Flt3L treatment on DC populations in spleen and lymph nodes during mBSA arthritis.

A) Representative FACS plots illustrating effects of Flt3L-treatment on cDCs and pDCs in the spleen of mBSA immunized mice. Cells were gated on the total mononuclear cell population. B) The relative increase (%) in splenic cDCs and pDCs after Flt3L-treatment (day 10, n = 15 and day 28, n = 23) compared to sham-treated controls (day 10, n = 15 and day 28, n = 27). C) Representative FACS plots illustrating effects of Flt3L-treatment on cDCs and pDCs in the draining lymph nodes of mBSA immunized mice. Cells were gated on the total mononuclear cell population. D) Relative increase (%) of DC populations after Flt3L-treatment (day 10, n = 10 and day 28, n = 22) in the lymph nodes compared with sham-treated controls (day 10, n = 6 and day 28, n = 27). The relative increase was calculated as described in material and methods. Data are presented as mean ± SEM and statistical significance was assessed using unpaired t-test. *P<.05, **P<.01, ***P<.001.