The mechanism research of lncRNA HOTAIR/miR-1/GOLPH3 axis regulated proliferation and migration in breast cancer

Background  lncRNA HOTAIR is frequently overexpressed in breast cancer tissues and is associated with the development of breast cancer. However, the role of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells ,remain largely unknown. 

Methods  We investigated the level of HOTAIR in breast cancer and its clinical pathological characteristics by bioinformatic methods.Then,We evaluated the effects of HOTAIR and miRNA-1 expression on the biological behavior of breast cancer cells by qPCR, Cell Counting Kit-8 (CCK-8) assay, Clonogenic assays, Transwell assay and Flow cytometry for cell proliferation, invasion migration and apoptosis, and cell cycle analysis. Finally,The target genes of the lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis were validated by Luciferace reports.

Results  The expression of HOTAIR in breast cancer tissues was significantly higher than that in normal breast tissues（P＜0.05）. Silencing of HOTAIR suppressed cell proliferation, invasion and migration, promoted apoptosis and induced G1 phase block in breast cancer（P＜0.0001）. We also verified the existence of paired sequences between miR-1 and HOTAIR, GOLPH3 and miR-1 by Luciferace reports (P＜0.001）.

Conclusions  The expression of HOTAIR was significantly elevated in breast cancer tissues. Reducing the expression of HOTAIR inhibited cell proliferation, invasion and migration of breast cancer cells and promoted apoptosis, and the mechanism was mainly the effect of lncRNA HOTAIR/miR-1/GOLPH3 regulatory axis on the biological behavior of breast cancer cells.