Determination of MIC for WT, ΔkpnO, ΔkpnOΩkpnO, ΔphoBKP, and ΔphoBKPΩphoBKP.

E-strips were used to determine the precise MIC for different group of antibiotics such as ceftazidime, cefepime, ceftriaxone, nalidixic acid, streptomycin, tetracycline, trimethoprim, vancomycin, amikacin, erythromycin, tobramycin, chloramphenicol, ciprofloxacin, colistin, norfloxacin, ofloxacin, polymyxin, rifampicin, sparfloxacin, and spectinomycin following the CLSI guidelines. Complementation restored the MIC values. Units for MIC values are µg/ml.aFold change is the ratio of MICs for WT and ΔkpnO or ΔphoBKP.