PSF

The Protein Structure Factory is established to characterize proteins encoded by the genes or cDNAs available at the Berlin Resource Center of DHGP. At a later stage, it may analyze various sets of input proteins selected by criteria of potential structural novelty or medical or biotechnological usefulness. It represents an integrative approach to structure analysis combining the computer-based analysis of genes by bioinformatics techniques, automated gene expression and purification of gene products, generation of a biophysical fingerprint of the proteins and the determination of their three-dimensional structures either in solution by NMR spectroscopy or in the crystalline state by X-ray diffraction.蛋白质结构工厂（Protein Structure Factory）旨在对德国人类基因组计划（DHGP）柏林资源中心所提供的基因或互补DNA（cDNA）编码的蛋白质开展结构表征工作。在后续研究阶段，该设施可针对依据潜在结构新颖性、医学或生物技术应用价值筛选得到的多组输入蛋白质进行分析。该设施采用整合式结构分析研究方法，融合了基于计算机的生物信息学基因分析技术、自动化基因表达与产物纯化流程、蛋白质生物物理指纹图谱构建，以及通过核磁共振波谱法（NMR spectroscopy）测定溶液状态下的蛋白质三维结构，或通过X射线衍射法（X-ray diffraction）测定晶体状态下的蛋白质三维结构。

搜集汇总

背景与挑战

背景概述

PSF（蛋白质结构工厂）旨在表征由柏林资源中心提供的基因或cDNA编码的蛋白质，未来可能基于结构新颖性或医疗价值筛选分析其他蛋白质。它采用整合方法，结合生物信息学、自动化基因表达与纯化、生物物理指纹分析，以及通过NMR或X射线衍射确定三维结构。