HIF1A regulated genes in MCF10A cells after treatment with Oncostatin M or control ligands

We employed a single-cell RNA sequencing approach to study the role of Oncostatin M (OSM) in inducing collective cell migration in MCF10A cells and its upregulation of HIF1A. To identify HIF1A-dependent transcriptional programs, we stably knocked down HIF1A using shRNA and treated cells with OSM. MCF10A breast epithelial cells stably expressing shHIF1A or control shSCR were grown in vitro and treated with Oncostatin M (OSM), epidermal growth factor (EGF), or a combination of EGF and interferon gamma (IFNG) for 24 hours prior to single-cell RNA sequencing.