Analysis of the mean intensity of expression of surface molecules and cytokines in monocytes CFSE+ and CFSE- after 15 hours of infection with Y strain and Col cl1.7.

Cells were double stained for the monocyte marker CD14 and the different molecules. Trypomastigotes from Y strain and Col cl1.7 were stained for CFSE. The analysis was performed by multiparametric flow cytometry, as described in Material and Methods. Results are expressed as the mean intensity of the molecule within the CD14+CFSE+ or CD14+CFSE- subpopulations and are indicated as average ± standard deviation for each analysis. Equal letters represent statistical difference (p < 0.05). Comparisons between groups were performed using One-Way Anova or Kruskal-Wallis test according to Kolmogorov-Sminorv normality test.
Analysis of the mean intensity of expression of surface molecules and cytokines in monocytes CFSE+ and CFSE- after 15 hours of infection with Y strain and Col cl1.7.