Transcriptome analysis of Physcomitrium patens wild type and PpRALF3 knockout lines under F. solani infection

According to our analysis, PpRALF2 and 3 KO genotypes were more resistant to phytopathogens than wild type plants and PpRALF1 knockout lines. To expand our understanding of the roles of PpRALFs in biotic stress response, we next sought to perform RNA sequencing (RNA-seq) analysis to compare transcriptome response of PpRALF3 KO and wild type plants during F. solani infection. Twelve paired-end RNA-Seq libraries were generated from three biological replicates of mock-inoculated and F. solani-infected wild type and PpRALF3 KO plants.