L.vannamei DEGs under acid stress

Litopenaeus vannamei Transcriptome under acid and alkline stresses. To understand the molecular mechanism of crustacean responses to low pH, we used RNA sequencing (RNA-seq) and investigated expression changes in whole gill transcriptomes of L. vannamei. We compared gill transcriptome of adult L. vannamei challenged by low pH (pH= 6.8) and a nonchallenged control group (pH= 8.0) at 1 h and 48 h, by using the Illumina HiSeq 4000 platform. This is the first study addressing the molecular response of the gills of L. vannamei to low pH stress at the whole-transcriptome level. The raw reads data can be obtained from the Short Read Archive of the National Center for Biotechnology Information under accession number SRP116151 or bioproject number PRJNA400112. The raw FASTQ file is too large for Mendeley storage limit, please refer to NCBI SRA.

本研究针对L. vannamei在酸性及碱性胁迫下的转录组进行了深入探讨，以揭示甲壳类动物对低pH值响应的分子机制。本研究通过RNA测序（RNA-seq）技术，分析了L. vannamei完整鳃部转录组在pH值为6.8的酸性胁迫条件下及pH值为8.0的非胁迫对照组下的表达变化。研究采用Illumina HiSeq 4000平台，分别在1小时和48小时后对比了两组鳃部转录组。此项研究系首次就L. vannamei鳃部对低pH值胁迫的分子响应进行全转录组水平的研究。原始测序数据可从美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）的短读序档案（Short Read Archive，SRA）中，通过访问号SRP116151或生物项目号PRJNA400112获取。由于原始FASTQ文件体积过大，超出Mendeley存储限制，请参考NCBI SRA。