Genome-wide expression changes in response to the treatment of anti-CD93 in mouse tumor endothelial cells

We isolated ECs from anti-CD93 antibody treated or control KPC tumor and performed RNA-seq to investigate genome-wide expression changes in response to the treatment of anti-CD93. Many of the genes affected by anti-CD93 were functionally related to cell adhesion, angiogenesis or vasculature development. Gene ontology analysis further confirmed that the transcriptomic changes by anti-CD93 were mainly related to biological pathways of angiogenesis, cell adhesion, or vasculature development. Total RNA was extracted and purified using QIAGEN RNeasy Mini Kit (Cat.74104) from tumor endothelial cells, which were isolated by FACS sorting (CD31+CD45- population) from established subcutaneous KPC tumor after 48 hours of antibody treatment. The mRNA libraries were constructed using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Cat. 634411). 150bp paired-end RNA sequencing was performed on Illumina NovaSEQ 6000 platform and the RNA-seq data were generated by the Genomics and Microarray Shared Resource at UCCC.