Remarkable Lung Heterogeneity at Single-Cell Resolution and the Role of Sex as a Biological Variable in Neonatal Hyperoxic Lung Injury

The goal of the project was to delineate sex-specific differences in the neonatal lung exposed to postnatal hyperoxia to model the pathophysiologic mechanisms in the human disease; bronchopulmonary dysplasia (BPD). C57BL6J neonatal mice were exposed to room air or hyperoxia from PND1-5 to 95% FiO2 or maintained in room air. Mice were euthanized at PND7. Single cell suspension were prepared for each of the four groups: male room air, female room air, male hyperoxia and female hyperoxia at the PND7 time point. Each sample was comprised of pooled lung tissue from three mice.本项目旨在阐明暴露于产后高氧环境的新生小鼠肺组织的性别特异性差异，以模拟人类疾病支气管肺发育不良（bronchopulmonary dysplasia, BPD）的病理生理机制。将C57BL6J品系新生小鼠分为两组：一组自出生后第1至5天（PND1-5）暴露于95%吸入氧分数（fraction of inspired oxygen, FiO2）的高氧环境，另一组则维持于室内空气环境；随后按性别将每组再分为两个亚组，最终形成雄性室内空气组、雌性室内空气组、雄性高氧组及雌性高氧组四个实验组。所有小鼠均于出生后第7天（PND7）实施安乐死。在该时间点，为四个实验组分别制备单细胞悬液，每个样本均由3只小鼠的肺组织混合制备而成。