Data from: Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

DNA sensing always has an open meadow of curiosity for biotechnologists
and other researchers. Recently, in this field, we have introduced an
emerging class of molecules containing azo and guanidine functionalities.
In this study, we have synthesized three new compounds (UA1, UA6 and UA7)
for potential application in DNA sensing in alcoholic medium. The
synthesized materials were characterized by elemental analysis, FTIR,
UV-visible, 1H NMR and 13C NMR spectroscopies. Their DNA sensing potential
were investigated by UV-visible spectroscopy. The insight of interaction
with DNA was further investigated by electrochemical (cyclic voltammetry)
and hydrodynamic (viscosity) studies. The results showed that compounds
have moderate DNA binding properties, with the binding constants range
being 7.2 × 103, 2.4 × 103 and 0.2 × 103 M−1, for UA1, UA6 and UA7,
respectively. Upon binding with DNA, there was a change in colour (a blue
shift in the λmax value) which was observable with a naked eye. These
results indicated the potential of synthesized compounds as DNA sensors
with detection limit 1.8, 5.8 and 4.0 ng µl−1 for UA1, UA6 and UA7,
respectively.