Summary of experimental timing and horse BALF EV characteristics across four different EV isolation methods.

This table includes information on EV isolation method characteristics across two different size exclusion chromatography approaches and two different centrifugation methods, as delineated by the columns. Pluses indicate the relative amount of each characteristic, with more pluses (++++) indicating that method had a higher value for that characteristic than other methods (+). Rows describe the following: (A) the time required for each EV isolation method (note that for SEC, EV isolation time is approximately 1 hour; however, cleaning the column for reuse for the next sample takes approximately 1 additional hour); (B) the resulting particle and (C) protein yield (reflective of yield from 20 mL unconcentrated horse BALF, where 0 indicates below the limit of detection for microBCA assay); (D) EV quality, as assessed through particle yields relative to protein yields and presence of EV and contaminant markers; and (E) number of proteins detected in at least 2 out of 3 samples within each isolation method.