Whole-genome RNA-deep sequencing of pancreatic islets of congenic Nidd/DBA mice

Here, we describe the QTL Nidd/DBA, a diabetogenic allele which was contributed by DBA and enhanced hyperglycemia and ß-cell loss. In order to re-address the issue of which variants on chromosome 4 are responsible for this trait, Nidd/DBA recombinant congenic lines were generated on an NZO background. Gene expression profiles of pancreatic islets were generated from congenic mice carrying the initial 13.6 Mbp fragment (RCS-I) of the Nidd/DBA locus. Six genes within the critical region were differentially expressed in pancreatic islets of homozygous Nidd/DBAD/D mice compared to homozygous Nidd/DBAN/N controls. Overall design: Analysis of pancreatic islets of 6-week old mice