RNA Sequencing Analysis between Control and CGA knockdown Multi-drug resistant Cells

The aim of this study is to compare the CGA Knockdown multi-drug resistant (MDR) cells (SGC7901/ADR and SGC7901/VCR) to the control cells. The total RNA of the indicated cells were extracted using Qiagen RNA Extraction Kit and RNA sequencing was performed. Significant differences in genes and signaling pathways were found between CGA knockdown and control MDR cells. This study provides a basis for applying RNA sequencing techniques to characterize targets in MDR cells. Overall design: Transcriptomic profiling between CGA siRNAs transfected SGC7901/ADR (or SGC7901/VCR) cells and scrambled control siRNA transfected SGC7901/ADR (or SGC7901/VCR) cells.