Illumina miRNA sequencing revealed Citrus adaptation to long-term B-toxicity through modulation of lateral root development by miR319

We report the application of Illumina sequencing for high-throughput profiling of miRNA in citrus root responded to long-term boron toxicity. We find miR319 is involved in citrus adapation to long-term boron toxicity via targeting a MYB gene, Ciclev10000756m.g.v1.0, which is homologus with several MYBs that modulate lateral root development in Arabidopsis. Citrus sinensis and C. grandis seedlings were sand cultured and treated with different levels of H3BO3 for 15 weeks. At the end of B treatments, root tips were used for total RNA extraction. small RNA libraries were then constructed and sequenced with an Illumina HiSeq 2500.