Contact residues involved in hydrogen bonding at the interface of native and mutated ACE based on PISA analysis.

The residue column gives the description of the chain, residue name, residue number and atom involved in hydrogen bonding. The distance column represents the calculated distance between the two interacting atoms. Bonding with Y465 is marked by bold. It was observed that transition from the native to the single followed by the double mutation results in systematic loss of H-bonding at the interface.