Cell landscape of Xenopus laevis at single-cell resolution

Single-cell mRNA sequencing (scRNA-seq) technologies are reshaping the current cell-type classification system. In previous studies, we built the mouse cell atlas (MCA) and human cell landscape (HCL) to catalog all cell types by collecting scRNA-seq data. However, systematically study for Xenopus laevis is still lacking. Here, we construct the Xenopus cell landscape with Microwell-seq protocols, which provides valuable resources for characterization of diverse cell populations of Xenopus laevis, and studying difference between vertebrates at single cell level. Overall design: Over 500,000 cells derived from adult Xenopus (one year old) dissociated tissues, including stomach (2 replicates), bladder (1 replicate), eye (1 replicate), intestine (2 replicates), kidney (2 replicates), lung (1 replicate), pancreas (1 replicate), spleen (1 replicate) and testis (2 replicates). Over 300,000 cells derived from adult Xenopus (one year old) dissociated tissues, including bone marrow (1 replicate), bladder (1 replicate), brain (2 replicates), eye (1 replicate), heart (1 replicates), liver (1 replicates), lung (2 replicates), muscle (1 replicate), ovary (1 replicate), oviduct (1 replicate), pancreas (1 replicate), skin (1 replicate) and spleen (1 replicate). Over 190,000 cells derived from Xenopus whole-body single-cell dissociation and profiling at the larval stage (NF48, NF54, NF59 and NF66 for each replicate).