Drosophila transcription factors, Séance, Ouija board and Molting defective, cooperatively control ecdysone biosynthesis. Drosophila transcription factors, Séance, Ouija board and Molting defective, cooperatively control ecdysone biosynthesis

RNA sequencing was performed to examine differential gene expression profiles in the ring gland of PG-specific Séance RNAi animals versus control. Overall design: Drosophila larvae with PG-specific knockdown of Séance and control animals were carefully staged at the larval L2/L3 molt. Ring glands were dissected at 44 hours L3. RNA isolated from ring glands were subject to RNA sequencing. Differential gene expression profiles were compared between control and RNAi animals.