Cotranscriptional Folding of a ZTP Riboswitch. Clostridium beijerinckii

RNAs can begin to fold immediately after emerging from RNA polymerase during transcription. Interactions between nascent RNAs and ligands during cotranscriptional folding can direct the formation of alternative RNA structures, a feature exploited by non-coding RNAs called riboswitches to make gene regulatory decisions. These sequencing datasets were generated using cotranscriptional SHAPE-Seq, which measures structural information of nascent RNAs during transcription, and combinatorial mutagenesis, which systematically perturbs riboswitch folding and measures RNA 3’ end distribution to determine function. The datasets describe the in vitro folding pathways of the C. beijerinckii ZTP riboswitch.