CRISPR and biochemical screens Identify MAZ as a co-factor in CTCF-mediated insulation at Hox Clusters

In this experiment, we sought to identify how the distribution of MAZ change in WT, MAZ HoxA(?5|6), and MAZ HoxD(?4|8) cells during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) Overall design: Examination of MAZ binding in embryonic stem cells and cervical motor neurons using ChIP-seq, by status of motif deletion in the cells: WT, MAZ HoxA(?5|6), and MAZ HoxD(?4|8)