Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells
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The fundamental interactions between plant cells and cryoprotectants during vitrification are understudied in the field of plant cryopreservation. Within this area research, real time cryoprotectant permeation into plant cells is even less documented. In this study, we monitor the real time permeation of individual cryoprotectants into rice callus cells when in mixtures with other cryoprotectants. Specifically, we use coherent anti-Stokes Raman scattering (CARS) microscopy to observe the permeation of individually deuterated DMSO, ethylene glycol, and glycerol in plant vitrification solution 2 (PVS2) by probing vibrational frequencies that correspond to C-D stretching modes of the cryoprotectant molecules. Additionally, we measure cell plasma membrane responses to PVS2 exposure using brightfield microscopy. We conclude that the permeation of PVS2 components into plant cells occurs faster than the first cell plasma membrane responses observed and therefore permeation and cell plasma memb..., , , # Data from: Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells
[https://doi.org/10.5061/dryad.np5hqc02q](https://doi.org/10.5061/dryad.np5hqc02q)
## Description of the data and file structure
This README file was generated on 2024-06-12 by Kylie Pearce.
## GENERAL INFORMATION
Author/Principal Investigator Information
Name: Nancy Levinger
ORCID: 0000-0001-9624-4867
Institution: Colorado State University
Email Address: [nancy.levinger@colostate.edu](mailto:nancy.levinger@colostate.edu)
Author/Co-investigator Information
Name: Kylie Pearce
ORCID: 0000-0002-8629-5932
Institution: Colorado State University
Email: [kylie.pearce@colostate.edu](mailto:kylie.pearce@colostate.edu)
Date of data collection: 2022-02-16 to 2023-01-26
Geographic location of data collection: Fort Collins, CO, United States and Aroura, CO, United State
Information about funding sources that supported the collection of the data: Main funding s...
创建时间：
2025-08-03
