Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells
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The fundamental interactions between plant cells and cryoprotectants during vitrification are understudied in the field of plant cryopreservation. Within this area research, real time cryoprotectant permeation into plant cells is even less documented. In this study, we monitor the real time permeation of individual cryoprotectants into rice callus cells when in mixtures with other cryoprotectants. Specifically, we use coherent anti-Stokes Raman scattering (CARS) microscopy to observe the permeation of individually deuterated DMSO, ethylene glycol, and glycerol in plant vitrification solution 2 (PVS2) by probing vibrational frequencies that correspond to C-D stretching modes of the cryoprotectant molecules. Additionally, we measure cell plasma membrane responses to PVS2 exposure using brightfield microscopy. We conclude that the permeation of PVS2 components into plant cells occurs faster than the first cell plasma membrane responses observed and therefore permeation and cell plasma memb..., , , # Data from: Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells [https://doi.org/10.5061/dryad.np5hqc02q](https://doi.org/10.5061/dryad.np5hqc02q) ## Description of the data and file structure This README file was generated on 2024-06-12 by Kylie Pearce. ## GENERAL INFORMATION Author/Principal Investigator Information Name: Nancy Levinger ORCID: 0000-0001-9624-4867 Institution: Colorado State University Email Address: [nancy.levinger@colostate.edu](mailto:nancy.levinger@colostate.edu) Author/Co-investigator Information Name: Kylie Pearce ORCID: 0000-0002-8629-5932 Institution: Colorado State University Email: [kylie.pearce@colostate.edu](mailto:kylie.pearce@colostate.edu) Date of data collection: 2022-02-16 to 2023-01-26 Geographic location of data collection: Fort Collins, CO, United States and Aroura, CO, United State Information about funding sources that supported the collection of the data: Main funding s...
在植物超低温保存领域，玻璃化过程中植物细胞与低温保护剂间的基础相互作用尚未得到充分研究；而该研究领域内，低温保护剂实时渗入植物细胞的相关文献更是鲜有记载。本研究针对混合条件下单一低温保护剂渗入水稻愈伤组织细胞的实时过程开展监测。具体而言，我们借助相干反斯托克斯拉曼散射（coherent anti-Stokes Raman scattering, CARS）显微镜，通过探测低温保护剂分子的C-D伸缩振动对应频率，观察了植物玻璃化溶液2（plant vitrification solution 2, PVS2）中氘代二甲基亚砜、乙二醇与甘油的渗入情况。此外，我们还通过明场显微镜测量了植物细胞质膜暴露于PVS2后的响应情况。本研究最终得出结论：PVS2各组分渗入植物细胞的速度快于观测到的初始质膜响应，因此渗入过程与植物细胞质膜…… # 数据来源：低温保护剂混合物可促进单一组分渗入活植物细胞 [https://doi.org/10.5061/dryad.np5hqc02q](https://doi.org/10.5061/dryad.np5hqc02q) ## 数据与文件结构说明 本README文件由Kylie Pearce于2024年6月12日生成。 ### 通用信息 #### 项目负责人/主要作者信息 姓名：Nancy Levinger ORCID：0000-0001-9624-4867 所属机构：科罗拉多州立大学（Colorado State University） 电子邮箱：nancy.levinger@colostate.edu #### 合作研究者信息 姓名：Kylie Pearce ORCID：0000-0002-8629-5932 所属机构：科罗拉多州立大学（Colorado State University） 电子邮箱：kylie.pearce@colostate.edu 数据采集日期：2022年2月16日至2023年1月26日 数据采集地点：美国科罗拉多州科林斯堡市与美国科罗拉多州Aroura市 支持本数据采集的资助来源信息：主要资助……
