Increased AIF-1-mediated p38 MAPK Phosphorylation in the Mid-secretory Endometrium Impairs Endometrial Receptivity in Women with Recurrent Implantation Failure

Summary answer: AIF-1 is significantly upregulated in the endometrial stromal cells of women with RIF, inhibiting cell proliferation and decidualization via the p38 MAPK phosphorylation, thereby reducing endometrial receptivity. Overall design: Using the T-HESC stromal cell line as the research model, transfection experiments were performed to construct two sample groups: the experimental group (transfected with an allograft inflammatory factor 1/AIF1 overexpression plasmid) and the control group (transfected with a nonsense sequence vector plasmid), followed by mRNA high-throughput sequencing analysis.