Additional file 21: Nucleotide sequence of pMCAS(Sync1-MSC16)dsRed in GenBank format. The sequence is compatible with common text editors or can be directly imported to appropriate software (Vector NT
(B, F, G) HEK293T ishXrn1cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with luciferase reporters containing insertions of the indicated lengths from t