miRNA profiling of control and ischemic samples in vitro and in vivo

miRNA microarray profiling of primary cortical neurons exposed to 4h oxygen and glucose deprivation (OGD) in vitro. RNA samples collected at 8h post-OGD terminaion. miRNAs were also profiled in mice exposed to 3- vessel occlusion model of stroke. RNA samples collected from contralateral (control) and ipsilateral (infarct) sides of the brain following 24h of reperfusion.针对体外原代皮层神经元的微小RNA（microRNA, miRNA）微阵列谱分析：将神经元暴露于4小时氧糖剥夺（oxygen and glucose deprivation, OGD）环境，于氧糖剥夺结束后8小时收集RNA样本。同时还开展了卒中三血管阻塞模型小鼠的微小RNA谱分析：于再灌注24小时后，从小鼠大脑的对侧（对照侧）与同侧（梗死侧）脑组织中采集RNA样本。