Marker gene sequencing of arbuscular mycorrhizal fungi associated with Taxus brevifolia and co-occuring Thuja plicata, Acer glabrum

We used marker gene sequencing to describe the arbuscular mycorrhizal fungal (AMF) communities associated with in-situ, mature western yew (Taxus brevifolia Nutt.) and co-occurring western redcedar (Thuja plicata Donn ex. D. Don) and Douglas maple (Acer glabrum var. douglasii) in the inland temperate rainforests of southern interior British Columbia. We sequenced the Small Subunit (SSU) genomic region using the AMF-specific primer pair WANDA/AML2 and Illumina sequencing.